Diphenylhydantoin microdetermination in serum and plasma by UV spectrophotometry

✍ Scribed by Jack E. Wallace; Horace E. Hamilton

• Publisher: John Wiley and Sons
• Year: 1974
• Tongue: English
• Weight: 452 KB
• Volume: 63
• Category: Article
• ISSN: 0022-3549
• DOI: 10.1002/jps.2600631133

✦ Synopsis

ma or urine following administration of a single 50-mg oral dose to humans".

An in v i m study was conducted to confirm the applicability of the method. A plot of plasma levels of hydroflumethiazide following a single intravenous injection to a beagle dog is shown in Fig. . The curve shows a typical biphasic pattern, with a half-life of 10 min for the a-phase and of 90 min for the @-phase. The amount of drug excreted in a 24-hr urine collection was 8.6 mg or 57% of the dose. The reason for incomplete excretion is not known at present. The analytical method was developed using human plasma and urine while the pilot experiment was conducted in dogs. However, dog plasma and uric were free of interfering impurities, as were the human fluids.

The procedure described in this paper is accurate and reproducible and has a high degree of sensitivity. It is quick and easy to carry out. One analyst can assay 20-25 plasma samples in a normal working day.