Department of Agriculture (K.J. K), Manhattan
Diphenolic compounds in cockroach hernolymph and cuticle were extracted with 1.2 N HCI, partially purified by alumina adsorption, and analyzed by liquid chromatography. Dopamine (DA) i s the major catecholamine in hemolymph of Periplaneta arnericana, Blatta orientalis, Blattella gerrnanica, Grornphadorhina portentosa, and Blaberus craniifer at adult ecdysis, while N-acetyldoparnine (NADA) predominates in hemolymph of Leucophaea rnaderae. In P. arnericana, NADA is the second most abundant catecholamine, while N-P-alanyldopamine (NBAD), norepinephrine (NE), 3,4dihydroxyphenylalanine, 3,4dihydroxyphenylethanol, 3,4dihydroxyphenylacetic acid, and 3,4dihydroxybenzoic acid occur in lesser quantities. Catecholamines occur mainly as acid labile conjugates in hemolymph. Doparnine, conjugated primarily as the 3-sulfate ester, increases in hernolymph from 0.1 to 0.8 m M during the last instar. Concentrations decrease by 75% in pharate adults, partially because of an increase in hemolymph volume. A second smaller peak of DA sulfate occurs after ecdysis followed by a rapid disappearance as the cuticle tans. A conjugate of catechol (0-dihydroxybenzene) is also present in relatively high concentrations at all ages examined. In cuticle, N-b-alanyl no repi neph ri ne accu rn u lates during the early period of adult tanning, while NBAD, NADA, N-acetylnorepinephrine, and DA increase more slowly. The N-0-alanyl and N-acetyl derivatives of DA Acknowledgments: Contribution No.