Diode laser based detection of single molecules in solutions

✍ Scribed by M. Sauer; K.H. Drexhage; C. Zander; J. Wolfrum

• Book ID: 103035541
• Publisher: Elsevier Science
• Year: 1996
• Tongue: English
• Weight: 394 KB
• Volume: 254
• Category: Article
• ISSN: 0009-2614
• DOI: 10.1016/0009-2614(96)00303-x

✦ Synopsis

Using a confocal microscope we studied bursts of fluorescence photons from single dye molecules that were iexcited at 632 nm with a CW diode laser or helium neon laser. The measurements were performed with new rhodamine der!vatives in ethylene glycol solution. The potential of this method for future construction of a low-cost instrument for single-molecule detection is pointed out. To demonstrate the utility of our new dyes in bioanalytical applications we st~owed the single-molecule detection of labeled oligonucleotides in water.