Diode array versus photomultiplier for measuring the UV-excited resonance Raman spectra of enzyme-substrate transients
✍ Scribed by P. R. Carey; L. R. Sans Cartier
- Book ID
- 102921029
- Publisher
- John Wiley and Sons
- Year
- 1983
- Tongue
- English
- Weight
- 563 KB
- Volume
- 14
- Category
- Article
- ISSN
- 0377-0486
No coin nor oath required. For personal study only.
✦ Synopsis
The construction of a multiplex system for recording the resonance Raman (RR) spectra of enzyme-substrate complexes in the near-UV region is described. The instrument is based on commercially available components consisting of a 2 W UV krypton ion laser, a triple spectrograph (in which the first two monochromators act as a zero dispersion, variable bandpass filter) and an intensified diode array. The performance of this system is compared witb that of a double monochromator with a photomultiplier as a detector. The enzyme-substrate systems chosen as examples for the comparison offer a difficult experimental challenge because they evolve rapidly and have poor optical homogeneity. In addition, a simple apparatus is described for recording the RR spectra of enzyme-substrate complexes in cryosolvents at temperatures as low as -50 'C.