Diode array detection of low level co-eluting species in high-performance liquid chromatography

✍ Scribed by N.H. Anderson; M.R. Gray; C.J. Hinds

• Publisher: Elsevier Science
• Year: 1990
• Tongue: English
• Weight: 214 KB
• Volume: 8
• Category: Article
• ISSN: 0731-7085
• DOI: 10.1016/0731-7085(90)80132-9

✦ Synopsis

A simple and sensitive method for detecting low levels (0.5 and 1.0%, w/w) of co-eluting species in HPLC has been developed. This method is based on the subtraction of normalized peak up-slope and down-slope spectra from that of the apex. Visual inspection of the resultant "difference spectra" allows for a qualitative judgement regarding the integrity of the peak under consideration.