## Abstract Eukaryotic initiation factor 5A (eIF5A) is the only protein in nature that contains hypusine, an unusual amino acid formed postβtranslationally in two steps by deoxyhypusine synthase and deoxyhypusine hydroxylase. Genes encoding eIF5A or deoxyhypusine synthase are essential for cell sur
Dimerization of the yeast eukaryotic translation initiation factor 5A requires hypusine and is RNA dependent
β Scribed by Petra M. Gentz; Gregory L. Blatch; Rosemary A. Dorrington
- Book ID
- 111312771
- Publisher
- John Wiley and Sons
- Year
- 2008
- Tongue
- English
- Weight
- 815 KB
- Volume
- 276
- Category
- Article
- ISSN
- 1432-1327
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Eukaryotic initiation factor 5A (eIF-5A) is the only protein in nature that contains hypusine, an unusual amino acid residue formed posttranslationally by deoxyhypusine synthase and deoxyhypusine hydroxylase. Although the eIF-5A gene is essential for cell survival and proliferation, the precise func
## Abstract A gene coding for a protein homologous to a translation initiation factor of eukaryotes, eIF5A, was cloned from __Methanococcus jannaschii__, a hyperthermophile with an optimum growth temperature of 85 Β°C. The protein was overexpressed, purified and crystallized. The crystals were obtai