Dihydrofolate reductase was synthesized in a batch system in the presence of the af6Nty ligand methotrexate, bound to various matrices. l k o types of gel were used: commercial methotrexate-agarose with pores inaccessible for translation machinery and methotrexate-POROS with pores easily accessible
✦ LIBER ✦
Dihydrofolate reductase synthesis in continuous culture using a methotrexate-resistant Escherichia coli
✍ Scribed by Poonam Nigam; Ibrahim M. Banat; Bridie A. Kelly; Roger Marchant
- Publisher
- Elsevier Science
- Year
- 1993
- Tongue
- English
- Weight
- 425 KB
- Volume
- 15
- Category
- Article
- ISSN
- 0141-0229
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## Abstract Pulse experiments in continuous‐culture are a valuable tool in microbial physiology research. However, inferences become difficult when the cell response is followed by monitoring many biochemical variables or when several types of perturbations are compared. Moreover, there is no objec