The aim of our work was to compare the mechanisms of resistance to aminopterin, inhibitor of the dihydrofolate reductase enzyme, between different Drosophila species and those described for cultured cells. Moreover we compared the systematic species divisions based on morphological traits and those
Dihydrofolate reductase and aminopterin resistance in Pneumococcus
β Scribed by Benedict, Stephen H. ;Gray, Thomas C.
- Book ID
- 104729214
- Publisher
- Springer
- Year
- 1973
- Tongue
- English
- Weight
- 739 KB
- Volume
- 122
- Category
- Article
- ISSN
- 0026-8925
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We have previously demonstrated systemic resistance to methotrexate (MTX) in transgenic mice carrying a foreign, mutant dihydrofolate reductase (DHFR, E.C. 1 S.1.3) gene. The new gene was introduced as a cDNA cloned into an expression vector driven by the simian virus 40 (SV40) early promoter. Previ
## Abstract We have used a methotrexateβresistant mouse 3T6 cell line (M50L3) that overproduces dihydrofolate reductase (DHFR) and its mRNA by a factor of about 300 to study the regulation of DHFR hnRNA synthesis. We have previously shown that when resting (G~0~) M50L3 cells are serum stimuled to r