Dihydrofolate reductase activity and loss of resistance in an antifolate-resistant subline of leukemia 11210 after discontinuation of treatment

Loss of folylpoly-γ-glutamate synthetase

Loss of folylpoly-γ-glutamate synthetase activity is a dominant mechanism of resistance to polyglutamylation-dependent novel antifolates in multiple human leukemia sublines

✍ Esti Liani; Lilah Rothem; Marlene A. Bunni; Clyde A. Smith; Gerrit Jansen; Yehud 📂 Article 📅 2002 🏛 John Wiley and Sons 🌐 French ⚖ 470 KB

## Abstract We have studied the molecular basis of drug resistance in human CCRF‐CEM leukemia cells exposed to high dose intermittent pulses of novel polyglutamatable antifolates that target various folate‐dependent enzymes. These include the dihydrofolate reductase (DHFR) inhibitors edatrexate, me