Digital Pulsed Force Mode : Determining Local Mechanical Properties of HeLa Cells

✍ Scribed by Michael J. Holzwarth; Alexander M. Gigler; Othmar Marti

• Book ID: 102866562
• Publisher: Wiley (John Wiley & Sons)
• Year: 2006
• Weight: 853 KB
• Volume: 8
• Category: Article
• ISSN: 1439-4243
• DOI: 10.1002/imic.200790065

✦ Synopsis

Many AFM measuring modes have been developed recently. To test the capabilities of these techniques, experiments on the most suitable sample system have been conducted. For example, phase-imaging techniques have been mainly tested on phaseseparating or dewetting polymer samples, while measurement modes based on the difference in electrical conductivity have been devised for semiconducting surfaces or samples with changes in work function. suited for AFM experiments due to their small height of about 2 µm. They were incubated under physiological conditions (37 °C, 5 % CO 2 ) in medium for three days. Before the actual measurement, the medium was rinsed-off with Phosphate Buff-