✦ LIBER ✦

Digital Holographic Microscopy : Label-free 3D Migration Monitoring of Living Cells

✍ Scribed by Björn Kemper; Patrik Langehanenberg; Angelika Vollmer; Steffi Ketelhut; Gert von Bally

Publisher: Wiley (John Wiley & Sons)
Year: 2009
Weight: 609 KB
Volume: 11
Category: Article
ISSN: 1439-4243
DOI: 10.1002/imic.200990081

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✦ Synopsis

Digital holographic microscopy (DHM) provides quantitative multi-focus phase contrast for high resolving investigations on reflective and transparent specimens that can be applied for label-free analysis of living cells. Results from application on mouse fibroblasts in collagen demonstrate that DHM enables minimallyinvasive automated 3D tracking of multiple cells without mechanical focus control.

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## Abstract For a precise manipulation of particles and cells with laser light as well as for the understanding and the control of the underlying processes it is important to visualize and quantify the response of the specimens. Thus, we investigated if digital holographic microscopy (DHM) can be u