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Diffusion of α-Chymotrypsin in Solution-Crowded Media. A Fluorescence Recovery after Photobleaching Study

✍ Scribed by Pastor, Isabel; Vilaseca, Eudald; Madurga, Sergio; Garcés, Josep Lluís; Cascante, Marta; Mas, Francesc


Book ID
121410652
Publisher
American Chemical Society
Year
2010
Tongue
English
Weight
138 KB
Volume
114
Category
Article
ISSN
0022-3654

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## Abstract The diffusion coefficient, __D__, and the proportion of mobile molecules, __p__, is measured for a fluorescently labeled polyelectrolyte in a multilayer film using fluorescence recovery after photobleaching (FRAP). The film was composed of poly(L‐lysine) (PLL) and hyaluronan (HA). The l