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Diffusion of Green Fluorescent Protein in the Aqueous-Phase Lumen of Endoplasmic Reticulum

✍ Scribed by Dayel, Mark J.; Hom, Erik F.Y.; Verkman, A.S.


Book ID
119412411
Publisher
Biophysical Society
Year
1999
Tongue
English
Weight
338 KB
Volume
76
Category
Article
ISSN
0006-3495

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The green fluorescent protein (GFP) was targeted to the endoplasmic reticulum (ER) of living plant cells using a virus‐based expression system. The signal peptide from the storage protein, patatin was fused to the amino‐terminus of the GFP while the ER retention signal KDEL was fused to the carboxy‐