Diffusion in cell-free and cell immobilising κ-carrageenan gel beads with and without chemical reaction

✍ Scribed by D. M. R. Mateus; S. S. Alves; M. M. R. da Fonseca

• Publisher: John Wiley and Sons
• Year: 1999
• Tongue: English
• Weight: 138 KB
• Volume: 63
• Category: Article
• ISSN: 0006-3592
• DOI: 10.1002/(sici)1097-0290(19990605)63:5<625::aid-bit13>3.0.co;2-0

✦ Synopsis

Diffusion into and from -carrageenan gel beads was studied, both in the absence and presence of bacterial cells, both with and without biochemical reaction. The solutes were indole, L-serine, and L-tryptophan. The reaction was that of indole and L-serine to give Ltryptophan. Established theory concerning diffusion of a single solute in cell-free gels was found to describe well the effect of the gel on diffusivity. Simultaneous diffusion of the three solutes resulted in lower diffusivities than those for individual solutes, suggesting the need to use multicomponent diffusion theory. The effect of cells on diffusion could only be accounted for by models assuming permeable cells. Diffusion with chemical reaction was reasonably well described by an effectiveness factor calculated using an effective diffusivity estimated from diffusion data without reaction.