Differentiation-promoting effect of 1-O (2 methoxy) hexadecyl glycerol in human colon cancer cells
✍ Scribed by Hongmei Wang; Sriram Rajagopal; Sharon Reynolds; Hokan Cederberg; Subhas Chakrabarty
- Publisher
- John Wiley and Sons
- Year
- 1999
- Tongue
- English
- Weight
- 127 KB
- Volume
- 178
- Category
- Article
- ISSN
- 0021-9541
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✦ Synopsis
Alkylglycerols are naturally occurring bioactive ether lipids found in great abundance in the livers of many marine species. In this study, we evaluated the differentiation-promoting potential of a methoxy substituted alkylglycerol-1-O (2 methoxy) hexadecyl glycerol (MHG)-to promote a more benign or differentiated phenotype in human colon cancer cells. Three cell lines with different biological and phenotypic properties were used. They were the moderately differentiated and growth factor-responsive Moser, the growth factor-unresponsive and malignant HT29, and the poorly differentiated and growth factorunresponsive HCT116. Treatment of these cell lines with MHG resulted in a downmodulation of cellular proliferation, a reduced propensity for anchorageindependent growth, and a reduced capacity in cellular invasion. Induction of the colon-associated and differentiation-related molecule carcinoembryonic antigen was also observed in the three cell lines. Induction of the transformation-sensitive and differentiation-related glycoprotein fibronectin was observed in the HT29 cells. It is concluded that MHG was biologically active and promoted a more benign or differentiated phenotype in these colon cancer cells. Since differentiation-inducing agents may possess chemoprevention properties, the use of MHG and the alkylglycerols in inducing differentiation or in chemoprevention of malignant diseases warrants further investigation.