Differentiation of the double membrane system during ascospore-maturation ofArthroderma vanbreuseghemiias revealed by periodic acid-alkaline bismuth staining
✍ Scribed by S. Tanaka; T. Fujigaki; S. Watanabe
- Publisher
- Springer Netherlands
- Year
- 1984
- Tongue
- English
- Weight
- 842 KB
- Volume
- 86
- Category
- Article
- ISSN
- 0301-486X
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✦ Synopsis
Asci ofArthroderma vanbreuseghemii were investigated by electron microscopy using periodic acid-alkaline bismuth (PABi) staining. At the early stage of ascosporogenesis, PABi-reactive substance was present homogeneously on both the inner and outer tracks of the double membrane system. Immediately after ascospore-delimitation, this substance gradually decreased and ultimately disappeared from the outer track. In contrast, the inner membrane was persistently loaded with the PABi-reactive material. These observations suggest that at this stage the outer membrane of the double membrane system begins to differentiate into the ascospore cell wall, utilizing its carbohydrate constituent.