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Differentiation of species and strains among filamentous fungi by DNA fingerprinting

✍ Scribed by Wieland Meyer; Anke Koch; Claudia Niemann; Birgit Beyermann; Jörg T. Epplen; Thomas Börner

Publisher
Springer-Verlag
Year
1991
Tongue
English
Weight
600 KB
Volume
19
Category
Article
ISSN
0172-8083

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✦ Synopsis

We have analyzed 11 strains and clones, representing five species (Penicillium janthinellum, P. citrioviridae, P. chrysogenum, Aspergillus niger, Trichoderma harzianum) and three genera of filamentous fungi, for the presence of hypervariable loci in their genomes by hybridization with simple repeat oligonucleotides and the DNA of phage M13. The oligonucleotide probes (CT)8, (GTG)5 and (GACA)4, as well as M13 DNA, are informative probes for fingerprinting in all genera and species tested. The probe (GATA)4 produced informative fingerprints only with the genomic DNA of A. niger. There was no similarity between the fingerprints originating from fungi of different genera and also little similarity between the fingerprints of different species belonging to the same genus. Fingerprints of strains of the same species differed only slightly from each other. Fingerprints of clones originating from one strain were identical. The results indicate that DNA fingerprinting is a powerful method to differentiate species and strains of filamentous fungi.

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