Differentiation of alloreactive versus CD3/CD28 stimulated T-lymphocytes using Raman spectroscopy: A greater specificity for noninvasive acute renal allograft rejection detection
✍ Scribed by Kristian L. Brown; Olena Y. Palyvoda; Jagdish S. Thakur; Sandra L. Nehlsen-Cannarella; Omar R. Fagoaga; Scott A. Gruber; Gregory W. Auner
- Publisher
- John Wiley and Sons
- Year
- 2009
- Tongue
- English
- Weight
- 287 KB
- Volume
- 75A
- Category
- Article
- ISSN
- 0196-4763
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✦ Synopsis
Abstract
Acute rejection (AR) remains problematic in renal transplantation. As a marker, serum creatinine is limited, warranting a more effective screening tool. Raman spectroscopy (RS) can detect T‐cell activation with high sensitivity. In this study we explore its specificity. Seventy‐five inactivated, 40 alloantigen‐activated, and 75 CD3/CD28‐activated T cells were analyzed using RS. CD3/CD28‐activated peak magnitudes (PM) were 4.3% to 23.9% lower than inactivated PM at positions: 903, 1031, 1069, 1093, 1155, 1326, and 1449 cm^−1^, with a difference in peak ratio (PR) observed at the 1182:1195 cm^−1^ position (0.91 ± 0.06 vs. 1.2 ± 0.01, respectively: P = 0.006). Differences in CD3/CD28‐ and alloantigen‐activated PM were observed at: 903, 1031, 1093, 1155, 1326, and 1449 cm^−1^, with no PR differences at the 1182:1195 cm^−1^ position (0.91 ± 0.06 vs. 0.86 ± 0.09: P = 0.8). Spectral signature separation of CD3/CD28—and alloantigen‐activated groups was 100% specific and sensitive. We conclude that RS can differentiate T cells activated by different stimuli with high sensitivity and specificity. © 2009 International Society for Advancement of Cytometry