✦ LIBER ✦

Differentiation in vitro of sea urchin micromeres on extracellular matrix in the absence of serum

✍ Scribed by Benson, Steve ;Chuppa, Sandy

Publisher: John Wiley and Sons
Year: 1990
Tongue: English
Weight: 592 KB
Volume: 256
Category: Article
ISSN: 0022-104X
DOI: 10.1002/jez.1402560212

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Previous investigations have demonstrated that differentiation in vitro of micromeres from sea urchin embryos requires the presence of serum. In this report we demonstrate that differentiation of micromeres, as judged by their ability to form spicules, will occur in the absence of serum if the cells are cultured on a reconstituted extracellular matrix prepared from Engelbreth‐Holm‐Swarm (EHS) tumors. These results suggest that a permissive environment provided by the EHS matrix is sufficient to induce micromere differentiation and may allow the isolation and characterization of the active component.

📜 SIMILAR VOLUMES

Role of the extracellular matrix on the

Role of the extracellular matrix on the growth and differentiated phenotype of murine colonic adenocarcinoma cells in vitro

✍ Jackie M. Walling; Melanie Blackmore; John A. Hickman; K. M. Stuart Townsend 📂 Article 📅 1991 🏛 John Wiley and Sons 🌐 French ⚖ 1023 KB

The growth and differentiation characteristics of MAC I 5 murine adenocarcinoma cells, derived from routine passage in vivo for growth in vitro on a plastic substrate (MAC15j cells), were compared under conditions in which the cells were seeded onto a substrate of type-I collagen which was either at

Effects of extracellular matrix molecule

Effects of extracellular matrix molecules on the growth properties of oligodendrocyte progenitor cells in vitro

✍ Jianguo Hu; Lingxiao Deng; Xiaofei Wang; Xiao-Ming Xu 📂 Article 📅 2009 🏛 John Wiley and Sons 🌐 English ⚖ 405 KB

## Abstract The extracellular matrix (ECM) is a component of neural cell niches and regulates multiple functions of diverse cell types. To date, limited information is available concerning its biological effects on the growth properties of oligodendrocyte progenitor cells (OPCs). In the present stu

Relative contributions of mechanical deg

Relative contributions of mechanical degradation, enzymatic degradation, and repair of the extracellular matrix on the response of tendons when subjected to under- and over- mechanical stimulations in vitro

✍ Paule Cousineau-Pelletier; Eve Langelier 📂 Article 📅 2009 🏛 Elsevier Science 🌐 English ⚖ 354 KB

## Abstract Tendon response to mechanical loading results in either homeostasis, improvement, or degeneration of tissue condition. In an effort to better understand the development of tendinopathies, this study investigated the mechanical and structural responses of tendons subjected to under‐ and