𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Differentiating effect of sodium butyrate on human hepatoma cell lines PLC/PRF/5, HCC-M and HCC-T

✍ Scribed by Hidetsugu Saito; Toshio Morizane; Tetsu Watanabe; Tatehiro Kagawa; Shingo Miyaguchi; Naoki Kumagai; Masaharu Tsuchiya

Publisher
John Wiley and Sons
Year
1991
Tongue
French
Weight
782 KB
Volume
48
Category
Article
ISSN
0020-7136

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

The in vitro effect of sodium butyrate (SB) on human hepatoma cell lines PLC/PRF/5, HCC‐M and HCC‐T was investigated. SB was added at the non‐toxic but cytostatic concentration of I mM. In all these cell lines, SB reduced cell proliferation and changed the morphology of the cells into a fibroblast‐like shape. In PLC/PRF/5, alpha‐fetoprotein production and c‐myc expression were inhibited. In contrast, gene expression of albumin, one of the normal liver‐cell products, Mid that of integrated hepatitis B virus genome, was increased. In HCC‐M and HCC‐T, c‐myc expression, which was enhanced In the naive state, was reduced. In HCC‐M, fos expression was inhibited but the expression of N‐ and K‐ras genes did not change. SB seemed to induce normal or mature properties of hepatocytes in human hepatoma cell lines.

📜 SIMILAR VOLUMES

Loss of butyrate-induced apoptosis in hu
Loss of butyrate-induced apoptosis in human hepatoma cell lines HCC-M and HCC-T having substantial Bcl-2 expression
✍ Hidetsugu Saito; Hirotoshi Ebinuma; Masahiko Takahashi; Fumihiko Kaneko; Kanji W 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 273 KB 👁 1 views

We have demonstrated that sodium butyrate induces differentiation in human hepatoma cells; however, recent studies have shown that this agent causes apoptosis in some types of cancer cells. In this study, we examined whether sodium butyrate causes apoptosis in the human hepatoma cell lines, HCC-M an