Differential transport requirements of HLA and H-2 class I glycoproteins

✍ Scribed by Jeff Alexander; J. Alan Payne; Richard Murray; Jeffrey A. Frelinger; Peter Cresswell

• Book ID: 104747292
• Publisher: Springer-Verlag
• Year: 1989
• Tongue: English
• Weight: 842 KB
• Volume: 29
• Category: Article
• ISSN: 0093-7711
• DOI: 10.1007/bf00375866

✦ Synopsis

Transport of human and mouse major histocompatibility complex class I glycoproteins has been examined in a transport deficient B-lymphoblastoid cell line x T-lymphoblastoid cell line (B-LCLxT-LCL) hybrid, 174 x CEM.T2 (T2). This cell line expresses no detectable endogenous HLA-B5 and reduced levels of HLA-A2 on its surface although these molecules are synthesized. In order to study this defect further, either HLA-Bw58 or HLA-B7 genomic clones were transfected into T2. Metabolic labeling and immune precipitation demonstrated biosynthesis of the Bw58 or B7 glycoprotein. However, like the endogenous HLA-B5 molecule, neither HLA-Bw58 nor HLA-B7 was expressed at the cell surface. The cloned genes were properly expressed on the surface of C1R, a control B-LCL. To determine if mouse class I alleles had the same transport requirements as the human class I glycoproteins, either mouse H-2D p or H-2K b class I genes were introduced into T2. Surprisingly, the H-2 class I glycoproteins were transported to the cell surface normally. These data suggest a fundamental difference between human and mouse histocompatibility antigens in their requirements for intracellular transport.