✦ LIBER ✦
Differential staining of the X-chromosome during meiosis of orthoptera by a silver impregnation procedure
✍ Scribed by José Fernandez-Piqueras; Antonio Rodriguez Campos; Carlos Sentis Castaño; Enrique Rojo Garcia
- Book ID
- 104725296
- Publisher
- Springer
- Year
- 1982
- Tongue
- English
- Weight
- 881 KB
- Volume
- 85
- Category
- Article
- ISSN
- 0009-5915
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✦ Synopsis
Differential staining of the X chromosome of Orthoptera, which normally cannot be distinguished from the autosomes during the more condensed stages of meiosis, has been achieved by a silver impregnation procedure involving a pretreatment with 2 x SSC at 60 degrees C. The results suggest that the saline citrate solution preferentially extracts proteins from the X chromosome. This method may be useful for distinguishing chromosome regions composed of facultative heterochromatin in Orthoptera.