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Differential solubilization of proteins, phospholipids, and cholesterol of erythrocyte membranes by detergents

✍ Scribed by Francis H. Kirkpatrick; Stanley E. Gordesky; G.V. Marinetti


Book ID
115722562
Publisher
Elsevier Science
Year
1974
Tongue
English
Weight
507 KB
Volume
345
Category
Article
ISSN
0005-2736

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Selective solubilization of proteins and
✍ Yu, John ;Fischman, Donald A. ;Steck, Theodore L. πŸ“‚ Article πŸ“… 1973 πŸ› Wiley (John Wiley & Sons) 🌐 English βš– 1015 KB

## Abstract Treatment of isolated human erythrocyte membranes with Triton X‐100 at ionic strength ⋍0.04 preferentially released all the glycerolipid and glycoprotein species. At low ionic strength, certain nonglycosylated polypeptides were also selectively solubilized. The liberated polypeptides we

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The protein assay method of I. Polacheck and E. Cabib (Anal. Biochem. 117, 311-314, 1981) has been modified to provide a general method for quantitating protein samples in the presence of detergents. Dilute detergent-solubilized membrane proteins, by using ribonucleic acid as a carrier, have been ef