Differential silver carbonate staining of sister chromatids in BrdU-substituted chromosomes
โ Scribed by V. J. Goyanes
- Publisher
- Springer
- Year
- 1978
- Tongue
- English
- Weight
- 344 KB
- Volume
- 40
- Category
- Article
- ISSN
- 0340-6717
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โฆ Synopsis
Differential staining of sister chromatids in BrdU-substituted human chromosomes is demonstrated by an ammoniacal silver carbonate procedure. With this method the chromosomes exhibit a subchromatid structure. Because proteolytic treatment indicated that the silver carbonate binds the chromosome proteins, changes of these components may be inferred in the BrdU-substituted chromosomes. Sister chromatid exchanges could be identified.
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