Differential sensitivity of mouse oocytes to colchicine-induced aneuploidy

✍ Scribed by John B. Mailhes; Zhi Ping Yuan

• Publisher: John Wiley and Sons
• Year: 1987
• Tongue: English
• Weight: 409 KB
• Volume: 10
• Category: Article
• ISSN: 0893-6692
• DOI: 10.1002/em.2850100208

✦ Synopsis

Unpublished results from our laboratory showed that colchicine increased the incidence of hyperploid mouse metaphase I1 (MII) oocytes when injected at the same time as human chorionic gonadotrophin (HCG). The objective of the present study was to determine whether the time of administering colchicine influenced the incidence of aneuploidy in MI1 oocytes. CD-1 mice were given pregnant mare's serum (PMS) and, 48 hr later, HCG. An intraperitoneal injection of 0.2 mg/kg colchicine was given at +4, + 2 , 0, -2 , or -4 hr relative to HCG.

Oocytes were collected 17 hr post-HCG and processed, and chromosomes were subsequently C-banded. The percentage of hyperploid oocytes was 0.77, 2.56, 5.71, 7.79, 3.54, and 2.70 for control, +4, + 2 , 0,-2, or -4 hr pre/post-HCG, respectively. Chi-square analyses of these data demonstrated that colchicine significantly increases the proportion of aneuploid oocytes, and that the relative sensitivity of colchicine-induced aneuploidy depends upon the time that this drug is administered relative to HCG.