Differential retention of lymphocyte subpopulations (B and T cells) on the microphase separated surface of polystyrene/polyamine graft copolymers

✍ Scribed by K. Kataoka; T. Okano; Y. Sakurai; T. Nishimura; S. Inoue; T. Watanabe; A. Maruyama; T. Tsuruta

• Publisher: Elsevier Science
• Year: 1983
• Tongue: English
• Weight: 420 KB
• Volume: 19
• Category: Article
• ISSN: 0014-3057
• DOI: 10.1016/0014-3057(83)90060-5

✦ Synopsis

To characterize the nature of differential retention of lymphocyte subpopulations (B and T cells) on polymer surfaces having microphase separated structures, the retention behaviour of lymphnode lymphocytes was studied as a function of contact time by using a column method: the cell suspension was passed through a "'column" packed with polymer-coated glass beads. Logarithm of proportion of effluent cells was found to be proportional to the contact time (r) of the cell suspension with the polymer surfaces. Au and A T are the proportional constants for B and T cells, respectively. Selectivity for lymphocyte subpopulations can be estimated by the ratio of AB/A T. The higher the value of this ratio, the higher is the differential retention of B cells. With albumin precoating, the microphase separated surfaces of polystyrene polyamine graft copolymer showed a dramatic increase in values of the AB/A T ratios, as high as 4.5, indicating that preferential retention of B cells over T cells took place on these surfaces. None of the homopolymer or random copolymer surfaces showed a value of AB/A T ratio higher than 2.0. A possible formation of microphase separated albumin layer is assumed on the surface of graft copolymer. This protein layer seems to play an important role in the differential retention of lymphocyte subpopulations.