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Differential regulation of Notch signal transduction in leukaemia and lymphoma cells in culture

✍ Scribed by Raffaella Chiaramonte; Elisabetta Calzavara; Francesca Balordi; Marta Sabbadini; Daniela Capello; Gianluca Gaidano; Anna Serra; Paola Comi; G. V. Sherbet

Publisher
John Wiley and Sons
Year
2003
Tongue
English
Weight
191 KB
Volume
88
Category
Article
ISSN
0730-2312

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✦ Synopsis

Abstract

The transduction of Notch signal plays an intricate role in cell differentiation and pathogenesis of haematological malignancies as well as in certain congenital conditions. We found no genomic changes in either gene in 34 leukaemic samples and 25 leukaemia and lymphoma cell lines. The functionality of Notch signalling was tested using HES1 gene activation. We show that Notch signalling is differentially regulated in T‐acute lymphoblastic leukaemia (ALL) and B‐lymphoma cells. The Notch pathway is intact in a majority of B‐lymphoma cell lines, but EBNA2, which mimics notch function, can occasionally activate the pathway. In contrast, the Notch pathway is constitutively active in T‐ALL. This is the first demonstration of a distinction between B‐lymphomas and T‐cell leukaemias in the functioning of the Notch‐signalling pathway. This might be related to their pathogenesis. © 2002 Wiley‐Liss, Inc.

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