Differential pulse polarographic determination of colchicine

✍ Scribed by Walter Holak; William M. Plank

• Publisher: John Wiley and Sons
• Year: 1980
• Tongue: English
• Weight: 286 KB
• Volume: 69
• Category: Article
• ISSN: 0022-3549
• DOI: 10.1002/jps.2600691223

✦ Synopsis

A differential pulse polarographic method for the analysis of colchicine-containing dosage forms is described. The reduction of the tropolone carbonyl is useful for quantitative analysis in that the relationship of the colchicine concentration to the current is linear over the concentration range of 0 to -50 pg/ml, with a slight negative deviation a t higher concentration. The procedure involves no preliminary treatment, is simple and specific, and is applicable to the assay of composite tablets, individual tablets, and injectable solutions. Polarography is conducted on a solution of colchicine in pH 1.81 Britton-Robinson buffer with 0.01% alkylphenoxy polyethoxyethanol. The quantitative analysis is achieved using the method of standard addition. A relative standard deviation of 3.2% was obtained for tablets. The results agree with those obtained using the USP XIX method.

Keyphrases 0 Colchicine-analysis by differential pulse polarography Polarography, differential pulse-analysis of colchicine 1436