Differential measurement with a microfluidic device for the highly selective continuous measurement of histamine released from rat basophilic leukemia cells (RBL-2H3)

Real-Time Monitoring of Histamine Releas

Real-Time Monitoring of Histamine Released from Rat Basophilic Leukemia (RBL-2H3) Cells with a Histamine Microsensor Using Recombinant Histamine Oxidase

✍ Shoko Iwaki; Masahito Ogasawara; Ryoji Kurita; Osamu Niwa; Katsuyuki Tanizawa; Y 📂 Article 📅 2002 🏛 Elsevier Science 🌐 English ⚖ 103 KB

To detect low levels of histamine, we developed a histamine microsensor using recombinant histamine oxidase. Histamine oxidase with a histidine tag was readily purified using a histidine affinity column. The enzyme showed higher catalytic activity on histamine than diamines (e.g., putrescine and cad