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Differential localization and expression of α and β isoenzymes of protein kinase c in the rat retina

✍ Scribed by Jun Kosaka; Akira Suzuki; Eiichi Morii; Shintaro Nomura

Publisher
John Wiley and Sons
Year
1998
Tongue
English
Weight
582 KB
Volume
54
Category
Article
ISSN
0360-4012

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✦ Synopsis

Expression and cellular localization of three isoenzymes of Ca 2ϩ -dependent protein kinase C (PKC␣, PKC␤, and PKC␥) in the adult rat retina were revealed by immunohistochemistry and in situ hybridization histochemistry with isoenzyme-specific antibodies and cRNA probes. Immunoreactivities and mRNA signals for PKC␣ were conspicuous in rod bipolar cells. A subgroup of amacrine cells expressed PKC␣. The cells in the ganglion cell layer also displayed PKC␣ gene products. Positive immunoreactivities for PKC␤ were localized as stripe patterns in the inner plexiform layer, corresponding to the stratification levels of axon terminals of cone bipolar cells. The somata of cone bipolar cells expressed PKC␤. Amacrine cells and retinal ganglion cells also displayed PKC␤ gene products. The results obtained by immunohistochemistry were confirmed with colocalization of mRNA signals for PKC␣ and PKC␤ on the somata. The cell membranes showed stronger immunoreactivities than did the cytoplasms for both PKC␣ and PKC␤. Neither immunoreactivities nor mRNA signals for PKC␥ were detected in all retinal regions. The differential roles of Ca 2ϩ -dependent PKC isoenzymes could be revealed in physiological defined retinal neurons.

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