✍ Scribed by Toshihiko Kondo; Masaya Kawakami
No coin nor oath required. For personal study only.
To demonstrate the concentration gradient distribution exactly in sample solutions during centrifugation, application of Svensson optics (Svensson, H. (1950) Acta Chem. &and. 4, 1329) to ultracentrifugal analysis is attempted and an instrument suited for a commercial analytical ultracentrifuge (Spinco Model E, Beckman) containing a Rayleigh interference optical system is devised. This instrument, which is composed of two pairs of glass plates and an assembly for synchronous tilt of these plates, is easily installed in the ultracentrifuge and is satisfactorily aligned using certain techniques including autocollimation method.
During high-speed centrifugation, the interference fringes display the sedimentation boundary of a solute as Gaussian curves, and the peak height is easily adjusted by a change in the tilt angle of the glass plates. The measurement accuracy is the same as that obtained using the usual interference optical system and much higher than that obtained using the schlieren optical system. The optimal range of protein concentration is 0.5-5 mg/ml. Various ways of applying this optical technique to ultracentrifugal analysis are suggested.
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