Differential induction of transcription of c-myc and c-fos proto-oncogenes by 12-O-tetradecanoylphorbol-13-acetate in mortal and immortal human-urothelial cells
✍ Scribed by Jan Skouv; Britta Christensen; Herman Autrup
- Publisher
- John Wiley and Sons
- Year
- 1987
- Tongue
- English
- Weight
- 633 KB
- Volume
- 34
- Category
- Article
- ISSN
- 0730-2312
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✦ Synopsis
The effect of the skin tumor-promoter TPA (12-0-tetradecanoylphorbol-13-acetate) on expression of cellular proto-oncogenes has been examined in cell lines derived from human urothelium. A single treatment with TPA (1 pg/ml) increased the transcription of clfos and c-myc proto-oncogenes at least 20-fold in the mortal cell line HU 1752. The induction was transient and was accompanied by a rapid but transient change in cell morphology. When immortalized cell lines were treated with TPA a similar rapid and transient morphological response was observed, but the TPA treatment only increased the level of clfos mRNA, suggesting that the normal regulation of c-myc transcription is altered in immortalized cells irrespective of their tumorigenic properties. The levels of c-Hams and c-Ki-rus mRNAs were unaffected by TPA treatment in all cell lines.