Differential induction of glucose-regulated and heat shock proteins: Effects of pH and sulfhydryl-reducing agents on chicken embryo cells
✍ Scribed by Sandra A. Whelan; Lawrence E. Hightower
- Publisher
- John Wiley and Sons
- Year
- 1985
- Tongue
- English
- Weight
- 909 KB
- Volume
- 125
- Category
- Article
- ISSN
- 0021-9541
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✦ Synopsis
Glucose-regulated and heat shock proteins are two subsets of eukaryotic stress proteins that can be induced differentially, simultaneously, and reciprocally. Two new inducers, low extracellular pH and 2-mercaptoethanol, that stimulate chicken embryo cells to synthesize glucose-regulated proteins rapidly were found. Two classes of cellular targets for mercaptoethanol were defined operationally, one dependent on and the other independent of protein synthesis. A new inducer of heat shock proteins, high extracellular pH, was found as well. Inductions by low and high extracellular pH were inhibited by actinomycin D but were insensitive to cycloheximide. Inductions of glucose-regulated and heat shock proteins are discussed in terms of changes in intracellular pH and sulfhydryl oxidation states.
Cells respond to a variety of adverse conditions by increasing the synthesis of a relatively small group of proteins (reviewed in Nover et al., 1984;Schlesinger et