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Differential expression of γ-aminobutyric acid-A receptor subunits in rat dorsal and ventral hippocampus

✍ Scribed by Evangelos Sotiriou; Costas Papatheodoropoulos; Fevronia Angelatou

Publisher
John Wiley and Sons
Year
2005
Tongue
English
Weight
490 KB
Volume
82
Category
Article
ISSN
0360-4012

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✦ Synopsis

Recent data demonstrate weaker g-aminobutyric acid (GABA)-ergic inhibition in ventral (VH) compared with dorsal (DH) hippocampus. Therefore, we examined possible differences regarding the GABA A receptors between VH and DH as follows: 1) the expression of the GABA A receptor subunits (a1/2/4/5, b1/2/3, g2, d) mRNA and protein and 2) the quantitative distribution and kinetic parameters of [ 3 H]muscimol (GABA A receptor agonist) binding. VH compared with DH showed: 1) lower levels for a1, b2, g2 but higher levels for a2 and b1 subunits in CA1, CA2, and CA3, the differences being more pronounced in CA1 region; in the CA1 region, the mRNA levels of a5 were higher, whereas those of a4 subunit were slightly lower; in dentate gyrus, the mRNA levels of a4, b3, and d subunits were significantly lower, presumably suggesting a lower expression of the a4/b3/d receptor subtype; and 2) lower levels of [ 3 H]muscimol binding, with the lowest value observed in CA1, apparently resulting from weaker binding affinity, insofar as the K D values were higher in VH, whereas the B max values were similar between DH and VH. The differences in the subunit expression and the lower affinity of GABA A receptor binding observed predominantly in the CA1 region of VH suggest that the a1/b2/g2 GABA A receptor subtype dominates in DH, and the a2/b1/g2 subtype prevails in VH. This could underlie the lower GABA Amediated inhibition observed in VH and, to some extent, explain 1) the higher liability of VH for epileptic activity and 2) the differential involvement of DH and VH in cognitive and emotional processes.

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