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Differential expression of theSesbania rostrataleghemoglobin glb3 gene promoter in transgenic legume and non-legume plants

✍ Scribed by Krzysztof Szczyglowski; Trevor Potter; Jon Stoltzfus; Susan Y. Fujimoto; Frans J. Bruijn

Book ID: 104615384
Publisher: Springer
Year: 1996
Tongue: English
Weight: 536 KB
Volume: 31
Category: Article
ISSN: 0167-4412
DOI: 10.1007/bf00019482

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✦ Synopsis

The involvement of the Sesbania rostrata glb3 gene promoter NICE (nodule-infected cell expression) element in root-enhanced expression of 5'-Srglb3-uidA-3'nos chimeric gene was investigated in transgenic Nicotiana mbacum plants. The full-length wild-type Srglb3 promoter directed root meristem-enhanced expression in transgenic tobacco plants. The expression pattern of nine selected Srglb3 promoter mutations in the NICE element was examined in transgenic tobacco plants and compared with the pattern observed in nodules of transgenic Lotus corniculatus plants. The results suggest that the highly conserved motifs in the NICE element play an important role in expression in roots of non-legume plants.

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