Differential expression of M-CSF, LIF, and TNF-α genes in normal and malignant rat glial cells: Regulation by lipopolysaccharide and vitamin D
✍ Scribed by I. Furman; C. Baudet; P. Brachet
- Publisher
- John Wiley and Sons
- Year
- 1996
- Tongue
- English
- Weight
- 768 KB
- Volume
- 46
- Category
- Article
- ISSN
- 0360-4012
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✦ Synopsis
The effect of 1,25-dihydroxyvitamin D, (1,25-(OH),D,) on the expression of macrophage colonystimulating factor (M-CSF), leukemia inhibitory factor (LIF), and tumor necrosis factor-a (TNF-a) genes in primary rat astrocytes and C6 glioma cells was examined. The results show that the hormone differentially regulates the cytokine mRNA in the two cell types. 1,25-(OH),D, augments M-CSF and LIF mRNA in C6 glioma cells, while lipopolysaccharide (LPS) has minimal effects. When LPS and 1,25-(OH),D, are used in combination, a strong synergistic effect upon the induction of M-CSF and LIF genes is observed. No TNF-a transcript has been detected in C6 glioma cells under any stimulus conditions used. In contrast, 1,25-(OH),D, has no pronounced effect on M-CSF, LIF, and TNF-a transcripts in primary astrocytes when used as a sole stimulus, while treatment with LPS strongly enhances the levels of the three cytokines. However, when 1,25-(OH),D, is used in combination with LPS, a partial reduction in LPSinduced levels of M-CSF and TNF-a mRNA is observed. The overall results indicate that genes coding for some inflammatory cytokines obey distinct regulatory mechanisms in C6 cells and in primary astrocytes. They also suggest that 1,25-(OH),D,, by altering the response of astrocytes to an inflammatory stimulus, could participate in the regulation of the CNS immune response.