𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Differential expression of HNF4α isoforms in liver stem cells and hepatocytes

✍ Scribed by Keguo Li; Haiquan Zhang; Yanjun Wang; Yun Wang; Meifu Feng

Publisher
John Wiley and Sons
Year
2006
Tongue
English
Weight
207 KB
Volume
99
Category
Article
ISSN
0730-2312

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

The hepatocyte nuclear factor (HNF4α) has been implicated in liver development and hepatocellular differentiation. The HNF4α expression in liver stem cells has not yet been fully investigated. Here, we describe the expression characterization of HNF4α in liver stem cells isolated from 2‐acetylaminofluorene/partial hepatectomy (AAF/PH) rat model by a combination of density‐gradient centrifugation and selective enzymatic digestion. The obtained cells were identified by flow cytometry (FCM) immediately and then by immunocytochemisty and reverse transcription‐polymerase chain reaction (RT‐PCR) after 5 d culture. The cultured stem cells were subjected to analysis of the expression of various HNF4α isoforms using RT‐PCR, Western blotting, and electrophoretic mobility shift assay (EMSA). Results showed that HNF4α isoforms, α1, α2, α7, and α8 were present in liver stem cells, which contrasted with only α1 and α2 expression in hepatocytes. The distinct expression patterns of HNF4α isoforms in liver stem cells may contribute to maintain the “stemness” of these cells. J. Cell. Biochem. 99: 558–564, 2006. © 2006 Wiley‐Liss, Inc.

📜 SIMILAR VOLUMES

Snail controls differentiation of hepato
Snail controls differentiation of hepatocytes by repressing HNF4α expression
✍ Carla Cicchini; Daniela Filippini; Sabrina Coen; Alessandra Marchetti; Claudio C 📂 Article 📅 2006 🏛 John Wiley and Sons 🌐 English ⚖ 346 KB

## Abstract Epithelial‐to‐mesenchymal transition (EMT) is a coordinated process, occurring both during morphogenesis and tumor progression, that allows epithelial cells to dissociate from initial contacts and migrate to secondary sites. The transcriptional repressors of the Snail family induce EMT

Differential regulation of constitutive
Differential regulation of constitutive androstane receptor expression by hepatocyte nuclear factor4α isoforms
✍ Jean Marc Pascussi; Agnes Robert; Amelie Moreau; Jeanne Ramos; Paulette Bioulac- 📂 Article 📅 2007 🏛 John Wiley and Sons 🌐 English ⚖ 498 KB

Constitutive androstane receptor (CAR; NR1I3) controls the metabolism and elimination of endogenous and exogenous toxic compounds by up-regulating a battery of genes. In this work, we analyzed the expression of human CAR (hCAR) in normal liver during development and in hepatocellular carcinoma (HCC)

Expression of HNF-1α and HNF-1β in vario
Expression of HNF-1α and HNF-1β in various histological differentiations of hepatocellular carcinoma
✍ Wang, Weiping; Hayashi, Yoshitake; Ninomiya, Toshiaki; Ohta, Kyosuke; Nakabayash 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 207 KB

Hepatic nuclear factor 1 (HNF-1) regulates genes in a hepatocyte-specific manner. It has been previously reported that the ratio of HNF-1 alpha and HNF-1 beta mRNA is related to histological differentiation hepatocellular carcinoma (HCC). In this study, the expression levels of the HNF-1 alpha and H

Expression of EGF receptor and FGF recep
Expression of EGF receptor and FGF receptor isoforms during neuroepithelial stem cell differentiation
✍ Kalyani, Anjali J. ;Mujtaba, Tahmina ;Rao, Mahendra S. 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 457 KB

To characterize the role of epidermal growth factor (EGF) and fibroblast growth factor (FGF) in regulating neuroepithelial stem cells differentiation, we have examined the expression of FGF, EGF, and their receptors by neuroepithelial (NEP) cells and their derivatives. Our results indicate that undi

In vitro differentiation of rat liver de
In vitro differentiation of rat liver derived stem cells results in sensitization to TNFα-mediated apoptosis
✍ Aránzazu Sánchez; Valentina M. Factor; Luis A. Espinoza; Insa S. Schroeder; Snor 📂 Article 📅 2004 🏛 John Wiley and Sons 🌐 English ⚖ 488 KB

Hepatic stem cells are activated after liver damage and have a critical role in tissue homeostasis and repair. Characterization of molecular and cellular events accompanying the expansion and differentiation of liver stem cells is essential for understanding the basic biology of stem cells and for f

Differential expression and biological a
Differential expression and biological activity of retinoic acid–induced TGFβ isoforms in embryonic palate mesenchymal cells
✍ Paul Nugent; Linh Ma; Robert M. Greene 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 273 KB 👁 1 views

The effect of retinoic acid (RA) on TGF-b mRNA expression and protein production in murine embryonic palate mesenchymal (MEPM) cells was examined by Northern blotting and TGF-b bioassay in association with TGF-b isoform-specific neutralizing antibodies. Heat or acid activation was used to distinguis