Differential Expression of Cytokine Genes in HIV-1 tat Transfected T and B Cell Lines
โ Scribed by V. Sharma; T.J. Knobloch; D. Benjamin
- Book ID
- 115576328
- Publisher
- Elsevier Science
- Year
- 1995
- Tongue
- English
- Weight
- 512 KB
- Volume
- 208
- Category
- Article
- ISSN
- 0006-291X
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Human macrophage and T cell lines were stably transfected with HIV-1 wild-type Tat or Tat mutants in the cysteine-rich region displaying trans-dominant negative effects on HIV-1 life cycle. The expression of HLA class I and class II molecules was not affected by wild-type Tat. Tat mutants, instead,
HIV-1 gene expression relies upon a complex machinery that is primarily controlled by two viral regulatory proteins, Tat and Rev. Rev is involved in regulating post-transcriptional events of HIV-1 gene expression. The Tat protein transactivates transcription from the HIV-1 5ะ long terminal repeat (L