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DIFFERENTIAL ENRICHMENT OF CELLS FROM EMBRYONIC RAT CEREBRA BY CENTRIFUGAL ELUTRIATION

โœ Scribed by Cara-Lynne Schengrund; Mary Ann Repman


Book ID
111170803
Publisher
John Wiley and Sons
Year
1979
Tongue
English
Weight
633 KB
Volume
33
Category
Article
ISSN
0022-3042

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Centrifugal elutriation of large fragile
โœ Adnan Nasir; Susan D. Reynolds; Peter C. Keng; Lynne M. Angerer; Robert C. Anger ๐Ÿ“‚ Article ๐Ÿ“… 1992 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 925 KB

In order to analyze the RNA populations present in different cells of very early embryos, we have developed a protocol to purify these large blastomeres using counterflow centrifugal elutriation (CCE). This procedure employs ethanol fixation to stabilize the cells against shear forces encountered du