✦ LIBER ✦

Differential effects of superoxide, hydrogen peroxide, and hydroxyl radical on intracellular calcium in human endothelial cells

✍ Scribed by D. Dreher; A. F. Junod

Publisher: John Wiley and Sons
Year: 1995
Tongue: English
Weight: 843 KB
Volume: 162
Category: Article
ISSN: 0021-9541
DOI: 10.1002/jcp.1041620118

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✦ Synopsis

Changes in intracellular Ca2+ homeostasis are thought to contribute to cell dysfunction in oxidative stress. The hypoxanthine-xanthine oxidase system (X-XO) mobilizes Ca2+ from intracellular stores and induces a marked rise in cytosolic calcium in different cell types. To identify the reactive 0, species involved in the disruption of calcium homeostasis by X-XO, we studied the effect of X-XO on [Ca2'], by spectrofluorimetry with fura-2 in human umbilical vein endothelial cells (HUVECj. The [Ca2'Ii response to X-XO was essentially diminished by superoxide dismutase (SOD) (200 U/mlj and catalase (CAT) (200 Uiml), which DNA damage) (de Mello Filho and Meneghini, 1985). Intracellular Ca2+ has emerged as a key regulator of cell function (Berridge, 1993). Thus, changes in Ca"

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