Differential effect of D-penicillamine on the cell kinetic parameters of various normal and transformed cellular types

✍ Scribed by L. Friteau; P. Jaffray; X. Ronot; M. Adolphe

• Publisher: John Wiley and Sons
• Year: 1988
• Tongue: English
• Weight: 511 KB
• Volume: 136
• Category: Article
• ISSN: 0021-9541
• DOI: 10.1002/jcp.1041360317

✦ Synopsis

Biomedical des Cordeliers, 75006 Paris (L.F., X.R., M.A.), and Laboratoire de Biochirnie A, HBpital Cochin, 750 14 Paris (f.).), France

The cell-growth-inhibitory and phase-specific effects of D-penicillamine on cell-cycle progression were investigated using cell-proliferation patterns, quantitative cell-cycle analysis by flow cytometry, and determination of the mitotic index and binucleate cell fraction of normal (rabbit articular chondrocytes, L 809, rabbit fibroblasts) and transformed (HeLa, L 929) cells. D-penicillamine treatment resulted in an inhibition of growth within a dose range of 5 x M to 7.5 x M. Examination of DNA by flow cytometric analysis revealed that rabbit articular chondrocytes were preferentially arrested in the GOil phase of the cell cycle, whereas the other cell lines were blocked in the G2+M phase; the increase in the proportion of cells with C2 + M DNA content was partially due to an enhancement of binucleate cells, resulting in a cytokinesis perturbation for HeLa and L 929 cells. These results showed that D-penicillamine affects cell proliferation through different events according to cell type.