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Differential dye-ligand chromatography as a general purification protocol for 2-keto-3-deoxy-6-phosphogluconate aldolases

✍ Scribed by Michael C. Shelton; Eric J. Toone


Book ID
103976940
Publisher
Elsevier Science
Year
1995
Tongue
English
Weight
321 KB
Volume
6
Category
Article
ISSN
0957-4166

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2-Keto-3-deoxy-6-phosphogluconate aldolase (EC 4.1.2.14) has been isolated from extracts of Zymomonas mobilis using differential dye-ligand chromatography and affinity elution with product/product analog. The one-step procedure gives an enzyme with specific activity 600 units mg-1. Only 1 out of 47