Use of differential dye-ligand chromatog
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R.K. Scopes
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Article
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1984
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Elsevier Science
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English
β 449 KB
2-Keto-3-deoxy-6-phosphogluconate aldolase (EC 4.1.2.14) has been isolated from extracts of Zymomonas mobilis using differential dye-ligand chromatography and affinity elution with product/product analog. The one-step procedure gives an enzyme with specific activity 600 units mg-1. Only 1 out of 47