✦ LIBER ✦

Differential distribution and function of microtubules and microfilaments in sea urchin coelomocytes

✍ Scribed by Edds, Kenneth T.

Book ID: 102806015
Publisher: Wiley (John Wiley & Sons)
Year: 1984
Weight: 911 KB
Volume: 4
Category: Article
ISSN: 0271-6585
DOI: 10.1002/cm.970040405

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✦ Synopsis

Sea urchin coelomocytes were used as a model system to investigate the distribution and role of microtubules and microfilaments in cell spreading and filopodial formation. By using immunoblot characterized antisera to tubulin and actin coupled with immunofluorescence techniques, cellular protrusions were seen to contain actin filaments but no microtubules. Cells depleted of MT's by cold and colcemid treatments could attach, spread, and transform to the filopodial morphology normally.

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