Differential deoxyribonucleic acid synthesis during microcycle conidiation inNeurospora crassa
✍ Scribed by Françoise Grange; Gilbert Turian
- Book ID
- 104760034
- Publisher
- Springer
- Year
- 1978
- Tongue
- English
- Weight
- 832 KB
- Volume
- 119
- Category
- Article
- ISSN
- 0302-8933
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✦ Synopsis
In conidia of Neurospora crassa germinating at 25 ~ C, DNA synthesis measured by incorporation of tritiated adenosine reaches a maximum soon after the outgrowth of the germ tube (6-7 h after inoculation). In conidia heat-treated at 46 ~ C (for 15 h), a maximum of incorporation of the DNA precursor occurs already 1 h after inoculation, then the incorporation progressively declines until the end of the heat-shock. When such conidia are shifted to 25 ~ C, a maximum of DNA synthesis occurs during the development of the presumptive conidiophore as at the outgrowth of normal germ tubes. This wave of DNA synthesis is followed by a second maximum of DNA synthesis, occurring only in the microcyclized cultures, when the premature differentiation of proconidia takes place. Prevention of this second wave of DNA synthesis with hydroxyurea or 5-fluorodeoxyuridine respectively reduces or fully inhibits such induced conidial differentiation.