Differential cellular expression of isoforms of inositol 1,4,5-triphosphate receptors in neurons and glia in brain

✍ Scribed by Sharp, Alan H.; Nucifora, Frederick C.; Blondel, Olivier; Sheppard, Carol A.; Zhang, Chuanyi; Snyder, Solomon H.; Russell, James T.; Ryugoand, David K.; Ross, Christopher A.

• Publisher: John Wiley and Sons
• Year: 1999
• Tongue: English
• Weight: 682 KB
• Volume: 406
• Category: Article
• ISSN: 0021-9967
• DOI: 10.1002/(sici)1096-9861(19990405)406:2<207::aid-cne6>3.0.co;2-7

✦ Synopsis

Inositol 1,4,5-trisphosphate receptors (IP 3 R) are mediators of second messenger-induced intracellular calcium release. Three isoforms are known to be expressed in brain, but their regional distributions and cellular localizations are little known. In order to better understand the roles of IP 3 receptor isoforms in brain function, a first step is to define their distributions. We have used affinity-purified antibodies directed against peptides unique to each isoform to determine their sites of expression in rat brain. Type 1 IP 3 R (IP 3 R1) is dramatically enriched in Purkinje neurons in cerebellum and neurons in other regions, consistent with previous studies. By contrast, IP 3 R2 is only detected in glia, whereas IP 3 R3 is predominantly neuronal, with little detected in glia. IP 3 R3 is enriched in neuropil, especially in neuronal terminals (which often contain large dense core vesicles) in limbic and basal forebrain regions including olfactory tubercle, central nucleus of the amygdala, and bed nucleus of the stria terminalis. In addition, IP 3 R1 and IP 3 R3 have clearly distinct time courses of expression in developing brains. These data suggest separate roles for inositol 1,4,5trisphosphate receptor isoforms in development, and for glial and neuronal function. The IP 3 R3 may be involved in regulation of neurotransmitter or neuropeptide release in terminals within specific nuclei of the basal forebrain and limbic system.