Differential absorption and esterification of dietary long-chain fatty acids by larvae of the dragonfly, Aeshna cyanea
✍ Scribed by Gregor Kirfel; Hans Komnick
- Publisher
- John Wiley and Sons
- Year
- 1999
- Tongue
- English
- Weight
- 181 KB
- Volume
- 40
- Category
- Article
- ISSN
- 0739-4462
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✦ Synopsis
In order to evaluate whether dietary long-chain fatty acids were differentially absorbed, Aeshna cyanea larvae received 5 ml oral doses containing combinations of two radiolabeled fatty acids at nearly equal radioactive and nmolar concentrations:
(1) 3 H-oleic and 14 C-palmitic acids; (2) 3 H-oleic and 14 C-stearic acids; and (3) 3 H-palmitic and 14 C-stearic acids. After 3 h or 1 day, hemolymph samples, midgut tissue, midgut contents and fat body tissue were collected and assayed for labeled fatty acids. The 3 H/ 14 C ratios indicated that there was a preference for absorption of the monounsaturated oleic acid over both saturated palmitic and stearic acids and that the shorter palmitic acid was absorbed at a higher rate than the longer stearic acid. There were also differences in the 3 H/ 14 C ratios of the various lipid classes of the midgut wall, hemolymph, and fat body that reflected differential esterifications and transport of these fatty acids. Arch. Insect Biochem. Physiol. 40:183193, 1999.