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Different Susceptibility of Protein Kinases to Staurosporine Inhibition : Kinetic Studies and Molecular Bases for the Resistance of Protein Kinase CK2

โœ Scribed by Flavio Meggio; Arianna Donella Deana; Maria Ruzzene; Anna M. Brunati; Luca Cesaro; Barbara Guerra; Thomas Meyer; Helmut Mett; Doriano Fabbro; Pascal Furet; Grazyna Dobrowolska; Lorenzo A. Pinna


Book ID
115131490
Publisher
John Wiley and Sons
Year
1995
Tongue
English
Weight
743 KB
Volume
234
Category
Article
ISSN
1432-1327

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โœ Joseph D. Buxbaum; Yadin Dudai ๐Ÿ“‚ Article ๐Ÿ“… 1988 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 529 KB

We have developed a microtiter-based assay for protein kinase activity which depends on the immobilization of substrate proteins to nitrocellulose. The technique makes use of a filtration manifold, allowing as much as a 10-fold increase in efficiency as compared to other protein kinase assays. We ha