Different progress of MDCK cell death after infection by two different influenza virus isolates
✍ Scribed by Zuzana Horníčková
- Publisher
- John Wiley and Sons
- Year
- 1997
- Tongue
- English
- Weight
- 224 KB
- Volume
- 15
- Category
- Article
- ISSN
- 0263-6484
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✦ Synopsis
The eect of in¯uenza strains A H 3 N 2 and B, isolated during the seasons of 1994 and 1995 in the Czech Republic, on MDCK cells was studied. Various concentrations of virus and conditions of nutrition were used during the cell culture. The virus replication and consequently fragmentation of genomic DNA together with cytotoxicity were investigated in the absence and presence of 10 per cent calf serum. Virus replication, regardless of type A or B, caused earlier DNA fragmentation in comparison to non-infected cells in tissue culture. The results showed that the in¯uenza B strain had a greater cytotoxic eect on MDCK cells than in¯uenza A. A higher infection dose of in¯uenza A virus accelerated the onset of apoptosis; conversely, a higher infection dose of in¯uenza B virus delayed the onset of apoptosis. The absence of serum enhanced the progress of in¯uenza-induced apoptosis in conditions in vitro.