Different neuronal Na+/K+-ATPase isoforms are involved in diverse signaling pathways
✍ Scribed by Larisa V. Karpova; Elena R. Bulygina; Alexander A. Boldyrev
- Publisher
- John Wiley and Sons
- Year
- 2010
- Tongue
- English
- Weight
- 154 KB
- Volume
- 28
- Category
- Article
- ISSN
- 0263-6484
- DOI
- 10.1002/cbf.1632
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✦ Synopsis
Abstract
Inhibition of rat neuronal Na^+^/K^+^‐ATPase __α__3 isoform at low (100 nM) ouabain concentration led to activation of MAP kinase cascade via PKC and PIP~3~ kinase. In contrast to ouabain‐sensitive __α__3 isoform of Na^+^/K^+^‐ATPase, an ouabain‐resistant __α__1 isoform (inhibition with 1 mM of ouabain) of Na^+^/K^+^‐ATPase regulates MAP kinase via Src kinase dependent reactions. Using of Annexin V‐FITC apoptotic test to determine the cells with early apoptotic features allows to conclude that __α__3 isoform stimulates and __α__1 suppresses apoptotic process in cerebellum neurons. These data are the first demonstration showing participation of ouabain‐resistant (__α__1) and ouabain‐sensitive (__α__3) Na^+^/K^+^‐ATPase isoforms in diverse signaling pathways in neuronal cells. Copyright © 2010 John Wiley & Sons, Ltd.