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Different mechanisms of hepatitis C virus RNA polymerase activation by cyclophilin A and B in vitro

โœ Scribed by Leiyun Weng; Xiao Tian; Yayi Gao; Koichi Watashi; Kunitada Shimotohno; Takaji Wakita; Michinori Kohara; Tetsuya Toyoda


Book ID
119193947
Publisher
Elsevier Science
Year
2012
Tongue
English
Weight
646 KB
Volume
1820
Category
Article
ISSN
0304-4165

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The nonstructural protein 5B (NS5B) of hepatitis C virus (HCV) is considered to possess RNA-dependent RNA polymerase (RdRp) activity and to play an essential role for the viral replication. In this study, we expressed the NS5B protein of 65 kd by a recombinant baculovirus. With the highly purified N